Fitc phalloidin染色
WebNov 9, 2024 · 3.3 Add phalloidin-conjugate working solution. Incubate at room temperature for 20–90 minutes. Tip: add DNA staining dye at this point. Tip: for vertebrate cells, it may be possible to add phalloidin … WebApr 13, 2024 · Phalloidin FITC (Sigma-Aldrich®, Vienna, Austria) was used in a concentration of 1:20 [5 μl Phalloidin and 95 μl PBS (1x)] and directly pipetted on the slides under wet conditions. Microscopic slides were exposed in dark conditions for 40 min and washed 5 times with PBS (1×) 10 min each. To diminish possible background …
Fitc phalloidin染色
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Webフルオレセインファロイジンは、緑色蛍光色素であるフルオレセイン(FITC)に結合した高親和性のF-アクチンプローブです。. • F-アクチンを選択的に染色. • 励起/発 … WebProduct overview. Phalloidin FITC Reagent (ab235137) is one of a series of phalloidin conjugates that selectively binds to actin filaments, also known as F-actin. Phalloidin …
WebApr 13, 2024 · Iodoacetyl-PEG-FITC,碘乙酰基(IA)官能化聚乙二醇(IA-PEG)是一种硫醇(-SH)基团反应性PEG衍生物,可用于通过其可用的硫醇基团修饰生物分子或其他材料。. 碘基很容易被硫醇基取代,形成稳定的碳-硫醇键。. FITC吸收波长为495nm,发射波长为515~520nm。. FITC基团 ... WebProperties. Description. Fluorescein isothiocyanate (FITC) labeled phalloidin binds and labels F-actin, but not G-actin. It is a green fluorescent dye, which can distinguish high …
Web荧光素-鬼笔环肽复合物是一种偶联绿色荧光染料荧光素 (FITC) 的高亲和力 F-actin 肌动蛋白探针。. • 选择性染色 F-actin 肌动蛋白. • 激发/发射波长:496/516 nm. • 优于抗体染色. • 适用于固定和透膜样品. 可获得优异的肌动蛋白染色研究结果. 鬼笔环肽是一种二环 ... WebThe Actin Cytoskeleton & Focal Adhesion Staining Kit consists of TRITC-conjugated phalloidin, anti-Vinculin & DAPI for the immunofluorescent staining of actin filaments in …
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Web查看其他细胞骨架染色产品 鬼笔环肽Phalloidin概述 鬼笔环肽是一种二环肽,属于致命性 鬼笔鹅膏 “死亡帽”蕈中分离出来的毒素家族中的一员,常用在固定细胞、透化的细胞和无 … bitterroot fishing access sitesWeb3.7 取 200μl 现配的 FITC-Phallodin 染色 ... FITC Phalloidin was purchased from Shanghai Mao Kang biotechnology Co., Ltd., ( Shanghai, China) [5] Cheng panLi et al. Alginate core–shell microcapsule reduces the DMSO addition-induced osmotic damage to cells by inhibiting cellular blebs. Chinese Journal of Chemical Engineering. bitterroot flower shopWebPlace each coverslip in a glass petri dish and extract it with a solution of acetone at ≤–20°C or 0.1% Triton X-100 in PBS. Leave for ~ 5 minutes. 2X Wash cells with PBS. When … datatable parent child rowsWebApr 12, 2024 · The fluorescent agent FITC-phalloidin, which stains F-actin filaments, was used to measure actin polymerization in sperm cells during capacitation. Sperm samples were collected by centrifugation (360 × g for 5 min), washed twice in PBS and fixed with 1% PFA in PBS (Santa Cruz Biotechnology Inc., Dallas, TX, USA) for 10 min. After fixation, … datatable power appWebiFluorTM 647鬼笔环肽染色与用于细胞分析的其他荧光染色完全兼容,包括荧光蛋白、Qdot® 纳米晶体和其他iFluorTM偶联物(包含iFluorTM偶联二抗)。另外,经本品结合后的F-actin仍能维持actin自身具有的许多生物学特性。且本品的结合没有物种差异性,适用性广泛。 datatable popup windowhttp://www.rx-8.com/chanpinzhongxin/203428.html datatable primarykey 複数WebTetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications. Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates, as evidenced by over 19,000 citations in the ... bitterroot flower tattoo