Greengate cloning
Webdestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression: Webday 1: amplify lvl0 unit, like promoter, terminator etc., perform GG reaction, transform; day 2: check E.coli by PCR, seed correct clones; day 3: prepare the plasmid, which can be …
Greengate cloning
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WebGreenGate is a simple and efficient cloning system for rapidly assembling plant transformation constructs . It is based on the Golden Gate method. Using the type IIS … WebJan 20, 2024 · Here, we adapted the GreenGate cloning vectors to create two destination vectors showing strong marking of cell membranes in either the whole root or specifically in the lateral roots. This system can also be used in both embryos and whole seedlings. As proof of concept, we follow both gene expression and anatomy in Arabidopsis …
WebJan 4, 2024 · Understanding the context-specific role of gene function is a key objective of modern biology. To this end, we generated a resource for inducible cell-type specific trans-activation based on the well-established combination of the chimeric GR-LhG4 transcription factor and the synthetic pOp promoter. Harnessing the flexibility of the GreenGate … Web57 rows · GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J …
WebOct 15, 2024 · One cloning kit, termed GreenGate, was designed to facilitate rapid cloning of . constructs using commonly used elements of plant transformation vectors [16]. Assembly . WebJul 19, 2024 · The driver lines were generated employing the fast and flexible GreenGate cloning system (Lampropoulos et al., 2013) but are compatible with any vector/transgenic line in which the expression of an effector is under the control of derivatives of the pOp promoter element (Moore et al., 1998). An important feature of our driver lines is the ...
WebNow, a new toolkit - aptly named GreenGate – offers all of those same advantages to researchers working in plant model systems. “The cloning is all done in vitro; it’s no different in mouse, human or plants,” explained Jan Lohmann of Heidelberg University. “What is different is the plasmids you use to bring this into your target organism.
WebGreenGate cloning system for plant transgenesis. Developed by Jan Lohmann and colleagues, GreenGate is a cloning system for the rapid assembly of plant transformation constructs. As the name suggests, GreenGate is based on the Golden Gate cloning method, but has been modified specifically to improve plant transgenesis. rbc canadian clearing codeWebNational Center for Biotechnology Information rbc canadian dividend fund - sr. a rbf266Weband versatile cloning system for the generation of plant transfor-mation vectors, which we named GreenGate. The GreenGate system allows rapid and efficient assembly of six … rbc canadian equity income-fWebThe characteristic of GreenGate cloning technology is sufficient to simultaneously construct a variety of effector expression vectors fused with different tags. On the basis of the … sims 3 framework setup file downloadWebApr 27, 2024 · An important prerequisite for a molecular toolkit is a universal and cost-effective cloning method that can be easily adopted by different laboratories. To this end, we generated a set of plasmids based on the GreenGate cloning system (Lampropoulos et al., 2013; Figure 1; Supplemental Table S1). This Golden Gate cloning system allows … rbc canadian bank linked gicWebThis plasmid is designed for Golden Gate cloning using the BsaI enzyme. These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were … sims 3 framework setup mod the simsWebThe plasmids used in this study were generated using either standard molecular cloning procedures (Sambrook et al., 1989) or by using the GreenGate Cloning system (Lampropoulos et al., 2013 ... sims 3 framework setup mods